Dynamics of <i>Bacillus</i> flotillin structures.

<p>A) overlay of a bright field image of a single <i>Bacillus</i> cells with the first frame of fluorescence (green signals) and of 9 trajectories, each having a different colour, of YFP tagged protein FloA, displayed over a time course of 8 seconds. B) individual mean squared displacement curves of the 9 particles observed (in corresponding colours) C) Example of about 50 tracks in 2 <i>Bacillus</i> cells, which are analysed in D) MSD curves for FloA; E) MSD curves of FloT for an experiment analogous to that shown in A) and C). F) weighted mean over all tracked MSD curves of FloA (3152 tracks, red curve) and of FloT (N = 1024, black curve). Error bars indicate the confidence of the mean values, (given by the weighted standard deviation, divided by the number of degrees of freedom). Dotted lines show the fits to the diffusion constants. G) Plot like in panel F), but differentiated into movement in the y-direction or in the x-direction for FloA-YFP; H) distribution of instantaneous velocities of FloA and along the perpendicular (v_x, blue bars) and longitudinal (v_y, red bars) cell axis, No = number. I) same as H) for FloT. For FloA, the tails of the distribution are visibly broader than for FloT, indicating a higher variance and therefore a higher diffusion constant of FloA.