Epidermal Growth Factor Receptor Mutation Enhances Expression of Cadherin-5 in Lung Cancer Cells - Fig 3

<p>A. Immunofluorescent analysis of CDH5 in A549 lung cancer cells transfected with empty vector (EV), wild type (WT) and mutant (L858R, DEL) EGFR genes. CDH5 was stained as green color and nuclei were stained as blue color. Scale bar 75 μm. B. The intensity of TGFBI in each cells was quantified and shown as the mean ± SD of three independent experiments. * denotes <i>p</i><0.05. C. CDH5 was determined by immunohistochemical (IHC) staining in mutant and wild type A549 lung cancer mouse xenografts. D. The expression of CDH5 was quantified and values of intensity are expressed as the mean ± SD of three independent experiments. * denotes <i>p</i><0.05. E. A549 lung cancer cells nude mice xenograft model. Data points represent the average of tumor volume± SEM (<i>n</i> = 4 in each group). A549 lung cancer cells were transfected with empty vector (EV), wild type (WT) and mutant (L858R, DEL) EGFR genes. * denotes that tumor volume between EV and DEL group was statistically significantly different at <i>p</i> < 0.05 by two-way ANOVA.