Additional file 2: Figure S2. of Ligation-free ribosome profiling of cell type-specific translation in the brain

Highly translated genes identified by ligation-free ribosome profiling are shifted to heavier polysomes. qPCR was performed with five probes on fractions isolated from a polysome profile from left frontal lobe brain tissue. a Genes found to be highly translated in ribosome profiling data, Snap-25 and Syt1, were found to be shifted to heavier polysomes; fractions 8 and 9. Genes found to be lowly translated, Tgfb1, Trpv6, and Pkd-1, were found to be most concentrated in lighter polysomes, fractions 4 and 5. b The polysome profile denotes from which portion of the profile fractions were obtained. (PDF 509 kb