Female worms are more resistant to a pro-oxidant challenge.

<p>(A) Total reduced thiol contents were determined by light absorption at 412 nm in females (closed circles) or males (open circles) homogenates after 1h pre-incubation of intact worms with 10 μM menadione in RPMI 1640. (B) Viability of females (closed circles) or males (open circles) was determined by light microscopy after 2h pre-incubation of intact worms with different concentrations of menadione in RPMI 1640 using a previously reported motility scale [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158429#pone.0158429.ref091" target="_blank">91</a>]. Females (closed triangle) or males (open triangle) viability incubated for 2h with 50 μM menadione and 1 mM n-acetylcysteine (NAC) were also determined. Data were expressed as mean ± SEM of four (A) or three (B) different experiments. Comparisons between groups were done by Student´s t-test (A) and two-way ANOVA and <i>a posteriori</i> Bonferroni (B). Fig (A): * <i>p</i><0.05. Fig (B): *** <i>p</i><0.001.