Sequence differences and correlation patterns between multiple MiR122 isomiRs detected in whitefish.

<p>(A) Nucleotide differences, marked with capital letters, within <i>pre-miR122</i> sequences from whitefish and Atlantic salmon. The data suggest that the isomiRs detected in the current study are products of two <i>mir122</i> loci, <i>mir122-1</i> and <i>mir122-2</i>. (B) (C) Hierarchical clustering (HCL) analyses (1-Pearson correlation) on the isomiRs 122-5p and 122-2-3p, respectively. Each row represents one isomiR between Control and MC-LR treated samples. Colors in the heatmaps represent normalized read count number of each individual isomiR. The separation of the MC-LR treated from Control samples is evident in the two cases. MiR122-5p isomiRs are clustered based on specific characteristics including the relative 3' endpoints and the length, and G/A difference in the mature sequence. These are discussed in the main text. Asterisks mark the archetype miRNAs.