The Complete Chloroplast Genome of Ye-Xing-Ba (<i>Scrophularia dentata</i>; Scrophulariaceae), an Alpine Tibetan Herb

<div><p><i>Scrophularia dentata</i> is an important Tibetan medicinal plant and traditionally used for the treatment of exanthema and fever in Traditional Tibetan Medicine (TTM). However, there is little sequence and genomic information available for <i>S</i>. <i>dentata</i>. In this paper, we report the complete chloroplast genome sequence of <i>S</i>. <i>dentata</i> and it is the first sequenced member of the Sect. Tomiophyllum within <i>Scrophularia</i> (Scrophulariaceae). The gene order and organization of the chloroplast genome of <i>S</i>. <i>dentata</i> are similar to other Lamiales chloroplast genomes. The plastome is 152,553 bp in length and includes a pair of inverted repeats (IRs) of 25,523 bp that separate a large single copy (LSC) region of 84,058 bp and a small single copy (SSC) region of 17,449 bp. It has 38.0% GC content and includes 114 unique genes, of which 80 are protein-coding, 30 are transfer RNA, and 4 are ribosomal RNA. Also, it contains 21 forward repeats, 19 palindrome repeats and 41 simple sequence repeats (SSRs). The repeats and SSRs within <i>S</i>. <i>dentata</i> were compared with those of <i>S</i>. <i>takesimensis</i> and present certain discrepancies. The chloroplast genome of <i>S</i>. <i>dentata</i> was compared with other five publicly available Lamiales genomes from different families. All the coding regions and non-coding regions (introns and intergenic spacers) within the six chloroplast genomes have been extracted and analysed. Furthermore, the genome divergent hotspot regions were identified. Our studies could provide basic data for the alpine medicinal species conservation and molecular phylogenetic researches of Scrophulariaceae and Lamiales.</p>