Representative fluorescence images of immunostained SG cells following 48 h cultivation on polymer coated glass plates.

<p>Representative fluorescent microscopic views of SGN and non-neuronal cells double labelled either with anti-neurofilament (NF, yellow) and anti-Vimentin (Vim, red) antibody or anti-p75 neurotrophic growth factor receptor (p75-NGFR, assigned in images as p75, yellow) and anti-Vim antibody. Nuclei were stained with DAPI. Positive staining against neurofilament and vimentin antigens were found in cells adhering on glass plates (<b>A</b>), PMTA (<b>C</b>), PDMAA (<b>E</b>) and PEtOx (<b>G</b>) demonstrating specific staining of SGN as well as fibroblasts and glial cells. To distinguish glial cells from fibroblasts, the glial cell specific anti-p75-NGFR antibody was used. Expression of p75-NGFR could be shown for glial cells adhering on glass plates (<b>B</b>), PMTA (<b>D</b>), PDMAA (<b>F</b>) and PEtOx (<b>H</b>). Images (<b>I</b>) and (<b>J</b>) represent assays incubated with the secondary antibodies goat anti-mouse conjugated with Alexa Fluor 488 (GaM 488), goat anti-chicken conjugated with Texas Red (GaCh TR) and goat anti-rabbit conjugated with Alexa Fluor 594 (GaRb 594) in the absence of the primary antibodies to demonstrate the specificity of the immune staining. Within the light exposure range between 1.5 and 2 sec no crossreactions between antigens and secondary antibodies as well among both secondary antibodies used for double staining were found.