Alignment of BET bromodomain mutants.

<p>(A) Secondary structure elements are shown at the top of the sequence alignment. Mutated residues are highlighted in red and studied mutations are listed. The conserved asparagine (N391in BRD3(2) numbering) is highlighted in yellow. The green dots represent the residues involved in binding with inhibitor JQ1 (PDB ID: 3ONI, 3S92, 3MXF). The residues underlined in blue are involved in PFI-1binding (PDB ID: 4E96). (B) Location of the mutations. Shown are the first (left) and second bromodomain of BRD2. The mutated residues are highlighted in ball and stick and the position of Cα atoms are shown as a sphere. The main structural elements are labelled.