Mutant coronins exhibit defects in sporozoite motility.

<p>Time-lapse images and randomly selected tracks of different parasite lines expressing endogenous coronin fused to mCherry with the indicated mutations in their putative actin-binding sites. Note that R24A, R28A moves in a similar manner to WT, while the other mutants move differently and exhibit bending movements so far not seen in any wild type or mutated sporozoite, most strikingly seen in the time lapse of a sporozoites expressing the R349E, K350E mutation. A small but discernable fraction of sporozoite expressing the K283A, D285A mutation in coronin can move in persistent circles. Scale bars: 5 μm (images), 10 μm (tracks).