Multiple sequence alignment and molecular phylogenetic analysis of the sand fly SALO protein family.

<p>(<b>A</b>) Multiple sequence alignment. Six different SALO-like proteins (LolSALOa-f) identified from the <i>B</i>. <i>olmeca</i> salivary gland cDNA library were aligned with similar proteins described in New World sand flies <i>Lu</i>. <i>longipalpis</i> (LJS, SALO) and <i>N</i>. <i>intermedia</i> (Linb-). Black background shading represents identical amino acids. Grey background shading represents similar amino acids. (<b>B</b>) The phylogenetic relationship among the sand fly SALO proteins is displayed. Whereas a large branch encompasses the more closely related homologs, other more divergent paralogs are also observed for all the three sand fly species. The evolutionary history was inferred based on the Whelan And Goldman model [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004771#pntd.0004771.ref062" target="_blank">62</a>]. Sand fly species are indicated by the different symbols in the legend on the right.