Effects of eupafolin on the LPS-induced NF-κB p65 and AP-1/c-fos nuclear translocation in RAW264.7 macrophages.

<p>The RAW264.7 macrophages were pretreated for 1 h with 60 μM eupafolin or with 30 μM of the MAPK inhibitors and were then treated with 1 μg/ml of LPS for 30 min. Western blot analysis (n = 3) was performed to evaluate the subcellular and nuclear localization of (A) phosphorylated (P-p65) and total p65 (t-p65) (total cell lysate) and (B) p65 and c-fos (nuclear fraction). GAPDH and PCNA (proliferating cell nuclear antigen) were processed in parallel as internal controls for protein loading. *<i>P</i><0.05 vs. the untreated control, ^†<i>P</i><0.05 vs. the LPS-treated cells. Immunofluorescence staining (n = 3) was performed to show the subcellular localization of (C) t-p65 and (D) c-fos. Bar = 50 μm.