Binding of FGF2-Luc with the overexpressed FGFR1.

<p>Living HEK293T cells transiently overexpressing human FGFR1 (transcript variant 2) were used as the receptor source. <b>(A)</b> Saturation binding of FGF2-Luc with the overexpressed FGFR1. Nonspecific binding data were obtained by competition with 250 nM of 6×His-FGF2. The measured bioluminescence data were expressed as mean ± SE (<i>n</i> = 3). The total binding data were fitted to Y = B_maxX/(K_d + X) + N_sX, specific binding data to Y = B_maxX/(K_d + X), and nonspecific binding data to a linear curve. <b>Inner panel,</b> Scatchard plot of the specific binding data. <b>(B)</b> Competition binding of wild-type or mutant FGF2s with the overexpressed FGFR1 using FGF2-Luc as a tracer. The measured bioluminescence data were expressed as mean ± SE (<i>n</i> = 3) and fitted with sigmoidal curves using the SigmaPlot10.0 software.