PAT is required for microneme secretion.

<p>(A) Localization of SS::GFP::TRAP in wildtype background sporozoites. (B) Localization of SS::GFP::TRAP in <i>pat</i>^{<i>ccp</i>.<i>PP</i>} background sporozoites. (C) Quantification of SS::GFP::TRAP fluorescence intensity in wildtype and mutant. (D) Quantification of speed of sporozoites. (E) Quantification of gliding motility of wildtype sporozoites activated with pluronic acid (F) Model of microneme secretion assay developed for TRAP detection by Western blot. (G) Western blot of 25k sporozoites of SS::GFP::TRAP in wildtype and promoter swap mutant using anti-GFP antibodies. Scale bars: 5μm. Data mean ± SEM.