RIG-I deficient BMDC are inefficient in antigen presentation to T cells.

<p>T cells were isolated from PR8 infected RIG-I^+/+ and RIG-I^-/- mice and co-cultured with BMDC generated from RIG-I^+/+ and RIG-I^-/- mice or vice versa. The levels of IFNγ production in CD8⁺ T cells were determined by flow cytometry (n = 8/group). (A) Representative dot plots showing IFNγ production. (B) Quantification of panel A. (C) Histograms showing the expression of costimulatory molecules CD86 and MHC-II on RIG-I^+/+ or RIG-I^-/- mice. BMDC were either mock infected (clear) or with IAV at a MOI of 0.5 (shaded). (D) Quantification of the mean fluorescent intensity (MFI) of CD86 upregulation on PR8 infected BMDC with relative to naïve control. (E) Quantification of MFI of MHC II upregulation on PR8 infected BMDC with relative to naïve control. (F-G) Susceptibility of RIG-I^+/+ or RIG-I^-/- BMDC to IAV infection. BMDC were infected overnight with an MOI of 0.5 and the infected population was identified by staining for viral hemagglutinin (HA) protein. (F) Representative dot plots showing HA+ BMDC. (G) Quantification of the frequencies of HA+ BMDC. (H) Comparison of MFI of CD86 upregulation on BMDC infected with different viruses. The upregulation of CD86 expression in infected BMDC was calculated by subtracting the MFI with MFI of naïve state. The data shown in panels A-B is from a representative experiment performed with n = 8 mice/group. The experiments were repeated twice. Data shown in panels C-H is a representative of 2 independent experiments done in triplicates. The values are expressed as mean ± SEM. * Denotes statistical significance at p<0.05 and ** denotes statistical significance at p<0.01.