The Notch and Wnt signalling pathways were affected by hypoxic conditions when human myoblasts were activated from G₀ arrest.

<p>The expression of <i>NOTCH1</i>, <i>NOTCH3</i> and <i>HES1</i> were up regulated in 1% O₂ for all three days, while <i>DLL1</i>, <i>JAG1</i> and <i>NUMB</i> were up regulated on day one only. However, the HES1 protein expression was induced in 21% O₂ on day 2 and 3. In the Wnt signalling pathway we observed an up regulation of <i>FZD7</i>, <i>LRP6</i> and <i>SFRP1</i> on day 1–3 in 1% O₂, whereas <i>WNT5B</i>, <i>AXIN2</i> and <i>CTNNB1</i> was up regulated only on day one. Accordingly, the protein expression of total and active non-phosphorylated β-catenin was up regulated in 1% O₂ in particular on day 1. Filled symbols: 1% O₂; Empty symbols: 21% O₂. Significance level: * p<0.05, ** p<0.01.