Characterization of subcellular fractions in control and MβCD treated myocytes.

<p>(1A) A representative blot demonstrating the subcellular distribution of α-actinin and cytochrome c oxidase subunit IV (cyt c ox) in time control myocytes (lanes 1 and 2) and myocytes treated with MβCD (lanes 3 and 4). (1B) Western blot demonstrating the effect of MβCD (lanes 3–4) on the subcellular distribution of caveolin-3 (cav-3); time control myocytes are in lanes 1–2. (1C) Summarized results showing the distribution of cav-3 in control and MβCD treated myocytes. Cardiomyocytes were treated with the cholesterol-depleting agent MβCD (5 mM) for 1 hour at room temperature. Subcellular fractions are: cytoskeletal (CySk), triton-insoluble (Insol), triton-soluble (Sol) and cytosol (Cyto). In the cytosol fraction caveolin-3 was not different and negligible compared to other fractions and therefore not given in the bar graph. *p < 0.05 vs. time control, n = 3–4/group.