The binding affinity between human insulin and analogues 3, 15 and 29 derived from SjIR-1, SjIR-2 and HIR, respectively, measured using the Octet RED system.

<p>Binding between human insulin and analogues 3 (A), 15 (B) and 29 (C) is shown at different concentrations (μM). The real time binding response (nm) was measured in seconds (sec). The parameters of the binding response (nm) and the KD value (M) of the binding between insulin and the analogues at different concentrations are shown in the tables on the right. The coefficient of determination (R^2) for all these interactions was close to 1.0, indicating a good curve fit.