LPS- and ASA-induced effects on GST activity.

<p>HepG2 cells were cultured to 80% confluence and treated with LPS alone (1μg/ml) for 24h. In some cases, the cells were also treated with 5 mM ASA for 24h alone or in combination with LPS or in the presence of NAC (10 mM) for 2h prior to LPS treatment, as described in Materials and Methods. GST activity in the mitochondria and PMS was measured as described before [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0159750#pone.0159750.ref013" target="_blank">13</a>]. Results are expressed as mean +/- SEM of at least three experiments. Asterisks indicate significant difference (*p≤0.05, **p ≤0.001 from control (C) and ɗ p≤0.05 compared to LPS- and ASA-treated cells).