Loss of <i>dac</i> reduces Ci^FL and <i>dppZ</i> levels in the MF and fails to potentiate <i>rdxZ</i> posterior to the MF.

<p>All panels show L3 eye imaginal discs, except F,J,N. (A-B´´´) Standard confocal section (A-A´) and cross-section (B´´´) through the dashed white line in B of a wild type disc stained with (A-B´) anti-β-Galactosidase to detect <i>rdxZ</i> (green in A-A´, B and white in B´), anti-Dac (red in A,B and white in B´´) and anti-Ci (blue in A,B; magenta in A´ and white in B´´´). Dashed yellow lines in B-B´´´ outline the domain where Dac is expressed at higher levels. (C-E´, G-I´, K-M´) <i>dac</i>^<i>3</i> clones marked by the presence of GFP (green in C,D,D´´,E-E´,G,H,I-I´,K,L,M-M´) and outlined by yellow dashed lines in D´,H´ and L´. Discs are stained with (C-E´) anti-Ci (magenta), (G-I´,K-M´) anti-β-Galactosidase to reveal (G-I´) <i>dppZ</i> (magenta) or (K-M ´) <i>rdxZ</i> (red in K and magenta in L-M´) and (K) anti-E-Cad (blue in K). Dashed white squares in C,G,K delimit the area shown in D-D´´, H-H´, L-L´, respectively. D´´ shows a merge of GFP signal relative to D. (F,J,N) Profiles of the intensity signals along the anterior-posterior (AP) axis of wild type (grey line) or <i>dac</i>^<i>3</i> mutant clones (orange line) for (F) Ci (at the levels of E or E´, respectively), or (J) <i>dppZ</i> (at the levels of I or I´, respectively) or (N) <i>rdxZ</i> (at the levels of M or M´, respectively). E-E´, I-I´ and M-M´ are outlined by dashed white rectangles in D,H,L. Plain white line in K’ indicates the MF. Scale bars represent 50μm.