Ablation of PrP^C does not affect APP proteolysis or Aβ peptide levels in APP_WT mice.

<p>Brain hemispheres from 32 week and 75 week old APP_WT/PrP^+/+ and APP_WT/PrP^-/- mice were subjected to two-step homogenisation, and the soluble proteins (SDS extracted) were subjected to SDS-PAGE and immunoblotting. (A) representative immunoblots of PrP^C and APP, with actin as a loading control. (B) Soluble (SDS extracted) sAPPα and sAPPβ detected by MSD ELISA and (C) the ratio of sAPPα to sAPPβ. (D) Soluble (SDS extracted) Aβ_38, Aβ₄₀ and Aβ₄₂ detected by MSD ELISA and (E) the ratio of Aβ₄₂ to Aβ₄₀. Data shown as mean ± SEM (n = 3–6). There was no significant difference between the APP_WT/PrP^+/+ and APP_WT/PrP^-/- at the same time point for any of the APP or Aβ peptides.