Homozygous bi-allelic mutant pollen tubes burst in pistils.

<p>(A to H) Pollen tube growth in pistils of wild-type (A,E), heterozygous T-DNA mutant <i>rupo+/-</i> (B,F), mono-allelic mutant (C,G) and bi-allelic mutant (D,H). White arrowheads indicate the putative positions of micropyles. All ovules were targeted by pollen tubes except for ovules from the bi-allelic mutant. Inserts in (E,F,G,H) are close-ups of representative pollen tubes in wild-type (E), <i>rupo+/-</i> (F), mono-allelic (G) and bi-allelic (H) stigmas. Red arrows in (H) indicate callus spots. (I,J,K,L) Bright-field images of representative pistils without aniline blue staining from wild-type (I), <i>rupo+/-</i> (J), mono-allelic (K) and bi-allelic (L) plants. Scale bars, 100 μm. (M) The percentage of ovules targeted by pollen tubes. n = 109 for wild-type pistils, n = 106 for <i>rupo+/-</i> pistils, n = 95 for mono-allelic pistils and n = 163 for bi-allelic pistils. (N) Pollen number per pistil before or after aniline blue staining. n = 79 (before staining) or 75 (after staining) for wild-type pistils, n = 74 or 107 for <i>rupo+/-</i> pistils, n = 59 or 89 for mono-allelic pistils, and n = 80 or 145 for bi-allelic pistils. Results are presented as mean ± s.e..