Optimization of TaqMan probe concentration for insulated isothermal PCR (iiPCR) assay of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc) race 4.

<p>Different concentrations (0, 50, 100, 150, 200, 250, 300, 350, 400, 450, and 500 nM) of probe were tested in the Foc iiPCR to evaluate the effects of TaqMan probe concentration on fluorescent signal production. The mean S/N ratio (fluorescent intensity_after/fluorescent intensity_before) of each reaction was plotted against the TaqMan probe concentration. Error bars represent the standard deviations from seven replicate reactions.