Identification of candidate miR-155 targets associated with M1 phenotype.

<p>(<b>A</b>) Fold Change (FC) vs. Mean Expression Value (MEV) plot of knockout (KO) M1 vs. wild-type (WT) M1 microarray data with miR-155 targets (high or moderate predicted targets and experimentally observed targets) that were down-regulated ≥2FC in WT M1 vs. WT M0 highlighted in red. 18 target genes were up-regulated more than 2 FC in KO M1 vs. KO M0. (<b>B</b>) Hierarchical clustering analysis of WT M0, WT M1, KO M0 and KO M1 samples based on expression of miR-155, IPA identified miR-155 dependent M1 Up genes from <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0159724#pone.0159724.g006" target="_blank">Fig 6</a></b> and top 18 miR-155 targets identified in (A). (<b>C</b>) <i>Inpp5d</i>, (<b>D</b>) <i>Mafb</i>, <b>(E</b>) <i>Tspan14</i>, and (<b>F</b>) <i>Bat5</i> expressions were determined by Real-Time PCR in wild-type (WT, n = <b>3</b>) and miR-155 knockout (KO n = <b>3</b>) bone marrow-derived macrophages <i>in vitro</i> activated in M1 or M2 conditions for 24 hours. Data shown is representative of 2–3 independent experiments. Gene expression is expressed as a percentage +/- SEM of the WT M0 condition. Unpaired t-test, *p<0.05, **p<0.005. (<b>F</b>)