Tip60 HAT activity restores EE mediated neuroadaptive benefits on MB morphology under APP neurodegenerative conditions.

<p>(A) Experimental paradigm for flies exposed to isolation (ISO) or environmental enrichment (EE) conditions. Newly eclosed adult fly progeny were exposed to a group of 30 flies (1:1 sex ratio) (EE), or housed individually (ISO) for 5 days. (B-D) Representative confocal images of adult MB visualized by mCD8-GFP and stained with axonal marker Fascillin II (Fas II) antibody from 5-day old adult fly expressing indicated transgenes driven by GFP;;OK107-Gal4 under ISO or EE condition. Anti-GFP staining used as marker to delineate MB in the adult brains. Anti-Fas II staining shows a/ß and r lobes in wild-type, APP and APP;dTip60^WT flies under ISO condition. (B’-D’) Same genotype flies in EE condition. (E) Quantification of area in the different genotypes and housing conditions in adult flies. Data represent the mean of 15 replicates with error bars depicting 95% confidence interval. Student t-test was used to determine statistical significance between different housing conditions within the same genotype. APP;dTip60^E431Q flies exhibited severely malformed MB structure and lack of structural consistency, and therefore are labeled as ND (non-determined) in the quantification. ** P < 0.01, *P < 0.05.