Transgene constructs and experimental plan.

<p>(A) Diagram of the two constructs used in the transgenic mouse lines for the generation of the reverse tetracycline-controlled transcriptional activator (rtTA) system (Tet-On system). <b>a.</b> The Pax8 promoter directs the expression of rtTA in renal tubular cells. <b>b.</b> The (TetO)₇/CMV-Ang-1-ZsGreen transgene is induced by a doxycycline (DOX)-bound rtTA. (B) Frozen kidney sections from 11 week-old P8TA; TETANG-1 mice receiving regular or DOX food, respectively, at three weeks of age for 8 weeks. Green fluorescent protein (ZsGreen) was expressed in all segments of the renal tubules in double positive transgenic mice administered DOX. Scale bar, 40 μm. (C) Representative immunoblot of Ang-1 expression in whole kidney lysates from mice of the indicated genotypes after 8 weeks of DOX administration. (D) The experimental plan used for the UUO study. CON, control.