Expression of ap-GnRHR isoforms (top two panels) in different <i>A</i>. <i>californica</i> tissues examined by RT-PCR.

<p>Primers used for the top panel amplify only ap-GnRHR-L; primers used for the second panel amplify both ap-GnRHR-L and ap-GnRHR-S. Negative controls (no RT) were RNA samples that have not been reverse transcribed (third panel). ap-Actin was used as a control to ensure the quality of RNA samples (bottom panel). The sizes of the PCR products are shown on the right. BCN, bag cell neurons; Abg, abdominal ganglia; Ceb, cerebral ganglia; P/P, pedal/pleural ganglia; Buc, buccal ganglia; Tail, ventral tail tissue; Osp, osphradium; SHD, small hermaphroditic duct; Hrt, heart; Ovt, ovotestis;—Cont, negative water control.