Schematic of <i>lacZ</i> fusions constructed for each gene tested.

<p><i>lacZ</i> fusions to RNA encoding the 5’-uORF (A) or to the coding sequence (CDS) of the downstream gene (B-D). The 5’-uAUG is either maintained as AUG (CDS), mutated to AUC (KO) or contains a premature stop codon (StartStop). The arrow indicates the in-frame AUG start codon defining the ORF providing expression of the <i>lacZ</i> reporter gene.