α-MSH induced phosphorylation of AMPK in differentiated L6 myotubes and in soleus explants dissected from lean and diet-induced obese (DIO) mice.

<p>A: Phosphorylation of AMPK in lean and DIO mice in basal conditions in soleus muscle explants (n=4). 2.B: α-MSH-phosphorylation of AMPK in lean and DIO soleus muscle explants (n=6). 2.C: Phosphorylation of AMPK after α-MSH-stimulation (100 nM) in differentiated L6 myotubes (n=4). AMPK phosphorylation is normalized to total AMPK. Unpaired one-tailed t-test was used to calculate statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001 vs. vehicle)