MIC13 localizes to inner mitochondrial membrane.

<p>(A) Representative images of mitochondria (marked by mito-GFP, green) and endogenous MIC13 (using MIC13 antibody, red) or MIC13-FLAG (marked by anti-FLAG) in control 143B cells. Merge shows the colocalization of mitochondria and MIC13. Scale bar 10μm. (B) Isolated mitochondria of control 143B cells were swelled by osmotic shock (OS) and then treated or untreated with Proteinase K (PK). Triton-x-100 (Tx100) was used to permeabilize all the membranes. TOM20, TIM23 and TFAM were used for outer membrane, inner membrane and matrix marker respectively.