Additional file 4: of A novel role for STOMATAL CARPENTER 1 in stomata patterning

Role of SCAP1 on stomatal genes transcript accumulations. (A) Transcript accumulation of stomatal markers SPCH, EPF2, MUTE and FAMA genes determined by quantitative PCR in manually dissected first two leaf primordia of 7 days-old wild type (Ler) and scap1-2 plants. Values represent the mean of three biological replicates (30 leaves/replica). (B-E) Transcript accumulation of stomatal markers EPF2, SPCH, MUTE and FAMA genes determined by quantitative PCR in 10 days-old DEX (or mock) treated wild type (Col) and (F-I) pro35S:SCAP1-GR (35S:SCAP1-GR) transgenic plants at different time point after treatment. Values represent the mean of two biological replicates. In all quantitative PCR ACTIN (ACT2) was used for normalization. Error bars = standard deviation. ** = P < 0.01 and * = P < 0.05 and two tails T Student test. Values in (A) are all not significantly different compared with the wild type. (JPG 348 kb