<i>In vivo</i> and <i>in vitro</i> characteristics of oncogene-transduced ID8 cells.

<p>(A, B, C) ID8, ID8-c-MYC, or ID8-KRAS cells (2 × 10⁶) were intra-peritoneal injected into C57/BL6 mice (ID8 group: n = 10, ID8-c-MYC group: n = 10, ID8-KRAS group: n = 10). ID8-KRAS mice were monitored every other day, and ID8 and ID8-c-MYC mice were monitored twice a week. Mice were sacrificed when the body weight reached 23 g. (A) The graph demonstrates Kaplan-Meier curve. The end point was when mice BW reached 23 g in each group. (B) The graph demonstrates that transition of BW in each group when mice BW reached. (C) The graph demonstrates the amount of ascites when BW in each group reached 23 g. Ascites weights were assessed using the following formula: (ascites weight) = (BW with ascites)—(BW without ascites). (D) ID8, ID8-c-MYC and ID8-KRAS cells were plated on 96-well culture plates at aconcentration of 5×10⁴ cells/ml and cultured for 48 h. The proliferation of ID8, ID8-c-MYC, and ID8-KRAS cells was assessed by cell proliferation assay. Each experiment was performed in triplicate and repeated 3 times. The average of three trials is shown. Error bars represent the mean ± SEM. (E) ID8, ID8-c-MYC, or ID8-KRAS cells (2 × 10⁶) were intra-peritoneal injected into C57/BL6 mice. Mice were sacrificed when the body weight reached 23 g. Disseminations were obtained from mice when sacrificed. Proliferation of tumor cells of each dissemination was assessed using Ki67 immunohistochemistry (IHC). Scale bars represent 50 μm and 30 μm at low and high magnification, respectively.