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Purification of truncated, FLAG- or His-tagged bovine αvβ6.

  • Gareth Shimmon (3142221)
  • Britta A. Wood (2918993)
  • Alison Morris (371631)
  • Valerie Mioulet (3142218)
  • Santina Grazioli (845653)
  • Emiliana Brocchi (845655)
  • Stephen Berryman (3142212)
  • Toby Tuthill (3142215)
  • Donald P. King (116717)
  • Alison Burman (395426)
  • Terry Jackson (363618)
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Publication date
August 2016
Icon DOI
10.1371/journal.pone.0160696.g003

Abstract

<p>(A) PAGE analysis of purified bovine αvβ6-FLAG. Lanes represent: crude cell culture supernatant (SN), the first wash step (W1), successive elution steps (E1–E3), and 2μg truncated human αvβ6 (h αvβ6). (B) PAGE analysis of purified, bovine αv-His/β6-His. Lanes represent: crude cell culture supernatant (SN), column flow through (FT), the first wash step (W1), and successive elution steps (E1–E5). (C) Western blot of αv-His/β6-His elution three (E3) using the anti-His antibody. M = molecular weight markers.</p

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