SLP-76 regulates <i>Ly49</i> gene transcription during early BM development.

<p><b>(A)</b> qPCR was performed for Ly49G2 and GAPDH (housekeeping gene) with RNA from sorted BM NK cells at various developmental stages [CD3ε⁻CD122⁺NK1.1⁻ (NKp), CD3ε⁻CD122⁺NK1.1⁺DX5⁻ (iNK), CD3ε⁻NK1.1⁺DX5⁺ (mNK)] and splenic CD3ε⁻NK1.1⁺DX5⁺ (NK). One representative of two independent experiments is shown. (<b>B)</b> qPCR for Ly49G Pro1 forward and reverse transcripts was performed on DX5^- (iNK) and DX5⁺ (mNK) BM NK cells from SLP-76 KO and littermate control mice. β-actin was used as a housekeeping gene. One representative of four independent experiments is shown. (<b>C)</b> ChIP for histone 3 lysine 9 (H3K9) acetylation was performed on splenic NK cells from WT and SLP-76 KO mice. The results were normalized as the percentage of the input (%input) from Ct values, and data are represented as mean % input ± SEM from three independent experiments. *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.001, and ns = not significant by paired student’s <i>t</i> test. See <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002526#pbio.1002526.s001" target="_blank">S1 Data</a> for raw data.