ELISA reactivity of antisera raised to different rCBF formulations against pure rCBF protein, outer membranes (OM) and whole meningococcal cells.

<p>Antisera from individual animals immunized with rCBF in various adjuvant and delivery formulations and from animals immunised with OM, were reacted in ELISA against <b>A) purified rCBF protein, B) MC58 OM and C) whole MC58 meningococcal cells</b>. The columns represent the geometric mean reciprocal ELISA titres (n = 4 or 5 animals per group) and the error bars represent the 95% confidence limits. No significant reactivity with rCBF, OM or whole cells in ELISA was observed with sera from sham-immunized animals or with normal mouse serum (absorbance values λ₄₅₀nm <0.1 for serum dilutions of 1/10). The * denotes that the responses were statistically higher than the corresponding sham-immunized animals (p<0.05), using an independent t-Test to compare differences between the mean values.