Lipodystrophy in PPARγ^-/F:MORE^+/Cre neonatal mice.

<p>Axillary, inguinal and subcutaneous WAT is significantly diminished and lacks mature adipocytes in PPARγ^-/F:MORE^+/Cre mice. (A-E) Skin was removed intact from euthanized P3 mice, restrained in a flattened position, fixed in 10% NBF and photographed from the deep aspect. PPARγ^-/F:MORE^+/Cre mice show greatly diminished axillary, thoracic and inguinal WAT (arrows in A) that contained wispy fibrovascular connective tissue, decreased mature adipocytes, and normal-appearing blood vessels (A, C, E, G, I) in place of well-formed WAT in controls (A, B, D PPARγ^-/F:MORE^+/+; F, H PPARγ^+/F:MORE^+/Cre;). Control (D) but not mutant (E) skin also showed refractile collections of subcutaneous adipose tissue that were diffusely distributed both superficial and deep (arrows in F and H) to the panniculus carnosus and were largely lacking in mutant skin (G and I). Dashed lines in A indicate the planes of microtome sectioning used for the histologic images presented in F-I. Long and short arrows in C indicate grossly lipemic and non-lipemic blood, respectively. Dashed rectangles in F and G indicate the areas shown at higher power in the insets. Scale bars: B, C 3 mm; D, E 1 mm.