Expression levels of stemness related markers and CCR7 in CD133⁺ pancreatic cancer stem-like cells.

<p>(A) Sorted CD133⁺ cancer cells and the total cells were cultured in the serum-free DMEM-F12 medium for 72h. The percentage of CD133⁺ in the total cell lines and sorted CD133⁺ were tested by FACS. The results displayed that purity of the CD133⁺ were 14.73% and 91.84%, respectively. (B) Oct-4, Sox-2, and CCR7 mRNA levels in total pancreatic cancer cells and in CD133⁺ and CD133⁻ cell fractions detected by RT-qPCR. Data were normalized to β-actin levels. Experiments were repeated three times with similar results. (C) CCR7 expression levels in total pancreatic cancer cells and in CD133⁺ and CD133⁻ cell fractions were detected by immunofluorescence staining (200×),(*P<0.05, **P<0.01, ***P<0.001).