AGGF1 is involved with autophagy activity.

<p><b>(A)</b> Western blot analysis showing that AGGF1 at different concentrations induces autophagy (increased LC3-II/β-actin and LC3-II/I; decreased p62 expression) in HUVECs under a normoxic condition for 4 h (<i>n</i> = 3/group). <b>(B)</b> AGGF1 (500 ng/ml) induces autophagy in HUVECs (representative images of LC3-GFP punctuate) (<i>n</i> = 6/group). Scale bar = 10 μm. <b>(C)</b> Representative electronic microscopic images of HUVECs treated with AGGF1 or control IgG showing detection of AGGF1-induced autophagy. Scale bar = 1 μm. <b>(D)</b> Western blot analyses for autophagy markers in cardiac tissues from <i>AGGF1</i>^<i>+/-</i> mice and wild-type <i>AGGF1</i>^<i>+/+</i> mice (<i>n</i> = 6/group). <b>(E)</b> Western blot analysis for autophagy markers in cardiac ECs isolated from <i>AGGF1</i>^<i>+/-</i> mice and wild-type <i>AGGF1</i>^<i>+/+</i> mice (<i>n</i> = 6/group). Underlying data are shown in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002529#pbio.1002529.s001" target="_blank">S1 Data</a>.