Additional file 3: Figure S1. of The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling

Increased endothelial marker expression during osteoblast differentiation of hiPSCs and differentiation of hiPSCs into ECs. (A) Quantitation of PECAM and VE-Cadherin gene expression by qPCR during 12 days of culture in osteogenic medium. (B) FACS analysis of mean percentage of cells expressing both PECAM and KDR on day 6 and 12 of osteogenic differentiation of three WT and three FOP hiPSCs lines with at least three replicates for each cell line. Error bars represent mean ± one SD. * p < 0.05, ** p < 0.01 by Student’s t test. (C) Mean percentage of cells co-expressing MSC markers (CD90+/CD73+/CD105+) on day 12 of osteogenic differentiation of three WT and three FOP hiPSCs lines. Error bars represent mean ± one standard deviation of at least three independent replicates for each of the three WT and four FOP cell lines. MSCs were not significantly increased in FOP hiPSCs during osteogenic differentiation by Student’s t test. (TIF 116 kb