Additional file 7: of TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide

Neurons are the only TLR4-expressing cells in ENS cultures. ENS cultures were stimulated for 24 h with LPS and costained for Hu C/D and TLR4. IL-6 antibody was also added to visualise macrophages (arrows). TLR4 was located exclusively in Hu C/D positive cells. Scale bars: 25 μm. (TIF 1806 kb