Arid3b^-/- B cells produce normal serum IgG and have no defect in response to LPS during ex vivo culture.

<p><b>A)</b> Peripheral blood was collected from control (N = 4) and <i>Arid3b</i>^<i>-/-</i> (N = 5) mice via cheek bleed and analyzed for total serum IgG levels. No difference was observed between control and <i>Arid3b</i>^<i>-/-</i> mice. <b>B)</b> Splenic B cells were isolated from control (N = 3) or <i>Arid3b</i>^<i>-/-</i> (N = 3) cohorts by negative selection of CD43 (Ly-48). Cells were stained with CFSE and cultured in LPS for 72 hours before analyzing by flow cytometry. Representative plots are shown. P values determined by unpaired students t-test. <b>C)</b> No difference was observed in the percentage of proliferating cells between control and <i>Arid3b</i>^<i>-/-</i> mice. <b>D)</b> Cultures were also analyzed for their ability to differentiate into plasma cells by cell surface markers B220 and CD138. <b>E)</b> No difference was observed in the percent of short lived plasma cells between control and <i>Arid3b</i>^<i>-/-</i> cultures.