Efficacy of CD123-specific CAR⁺ T cells for the treatment of B-ALL in a murine model.

<p><b>A)</b><i>In vitro</i> lysis of B-ALL cell lines by CD123-specific CAR⁺ T cells measured with a 4 hour chromium release assay <b>(B)</b> Schematic of the RCH-ACV B-ALL xenograft model. The experimental design is similar to that shown in 5A, but T cells and cytokines were given on days 7, 14 and 21, with imaging weekly. <b>(C)</b> BLI imaging of the CAR-treated and untreated groups on day 28. Images are displayed as in 5B. <b>(D)</b> Luciferase activity measured by BLI in the CAR-treated group compared with the untreated group. <b>(E)</b> Kaplan-Meier curves display the survival analysis of xenograft mice treated with CD123-specific CAR T cells compared with untreated mice. ** p < 0.01.