Tau and 14-3-3ζ forms a high molecular weight complex when co-expressed in M17 human neuroblastoma cells.

<p>Cells transfected with either Flag-tau or Myc-14-3-3ζ or co-transfected with Flag-tau and Myc-14-3-3ζ were subjected to OmniPrep density gradient centrifugation. Fractions were collected and analyzed by Western blotting and immunoprecipitation. <i>A</i>, Western blot of fractions corresponding to cells transfected with Flag-tau alone. <i>B</i>, Western blot of fractions corresponding to cells transfected with Myc-14-3-3ζ alone. <i>C</i>, Western blot of samples corresponding to cells co-transfected with Flag-tau and Myc-14-3-3ζ. Based on blot band intensities, the relative amount of Peak 1 (sum of fractions 1–4) and peak 2 (sum of fractions 5–8) was calculated and is expressed as the % of total (sum of fractions 1–8). <i>D</i>, Immunoprecipitation. Immunoprecipitation was carried out using fraction # 6 from panel <i>C</i>.