Chromosomal-break assay and association with the centromere histone H3.

<p>(a) Karyotype analysis of <i>D</i>. <i>bruxellensis</i> transformants carrying <i>CEN1</i> and <i>CEN2</i> plasmids integrated into the genome by PFGE. Y879 and Y997 strains were used as controls. Transformants with changed karyotypes are arrowed. (b) Co-immunoprecipitation with Cse4 antibodies. ChIP samples were used as a template for PCR performed at two annealing temperatures (64 and 65°C) using specific primers for <i>CEN1</i> (OP98 and OP99) and the sub-cloned fragment of <i>CEN2</i> (<i>CEN2-5</i>, primers SW9 and SW10). Samples: 1) 1 kb ladder; 2) ChIP Ca antibodies with Y879 chromatin (primers OP98 and OP99); 3) ChIP Ca antibodies with Y879 chromatin (primers OP98 and OP99); 4) ChIP Mouse Ig, negative control antibody with Y879 chromatin (primers OP98 and OP99); 5) no antibody, negative control with Y879 chromatin (primers OP98 and OP99); 6) 1 kb ladder; 7) input of ChIP assay, Y879 chromatin (primers OP98 and OP99); 8) 1 kb ladder; 9) ChIP Ca antibodies with Y879 chromatin (primers SW9 and SW10); 10) ChIP Ca antibodies with Y879 chromatin (primers OP98 and OP99); 11) 1 kb ladder; 12) input of ChIP assay, Y879 chromatin (primers SW9 and SW9); 13) input of ChIP assay, Y879 chromatin (primers OP98 and OP99); 14) ChIP Mouse Ig, negative control antibody with Y879 chromatin (primers SW9 and SW10); 15) no antibody, negative control with Y879 chromatin (primers SW9 and SW10); 16) ChIP Mouse Ig, negative control antibody with Y879 chromatin (primers OP98 and OP99); 17) no antibody, negative control with Y879 chromatin (primers OP98 and OP99); 18) 1kb ladder.