Effect of irradiated cells on angiogenesis in tumor formed out of bystander cells.

<p>(A) Tumor implanted with 1x10⁶ WEHI 164 cells (control) or tumor implanted with 1x10⁶ WEHI 164 unirradiated cells and 15 Gy irradiated 1x10⁶ WEHI 164 cells (mixed). At 11^th day of implantation, fluorescence imaging of animals were performed as mentioned in materials and methods. Representative images of animals from each group are shown. (B) Fluorescence intensity signal of animals (n = 6) was quantified. *significantly different than control at p<0.05. (C) Immuno-histochemistry of CD31 expression in tumor samples. Green areas indicate vasculature regions. Scale bar: 20 μm.