Inhibition of the PI3K/Akt pathway promotes PEDV replication.

<p><b>A.</b> Vero cells were pretreated with LY294002 (10 μM) 1 h prior to PEDV infection. Cell lysates were prepared after 6–10 h of infection and then analyzed using Western blotting. <b>B.</b> Quantification of viral titers using TCID₅₀ assays. <b>C.</b> Quantification of viral RNA copies using real-time PCR. Quantitative data are shown as mean ± SD (**p < 0.001) of three independent experiments.