Survival after bacterial challenges.

<p>(A) During the first septic assault, after CLP and prior to <i>P</i>. <i>aeruginosa</i> instillation (D5), 28% of CLP-operated mice (22/80) died. During this period, 100% of Sham-operated mice (n = 29) survived. (B) At D5 post CLP, mice were challenged with an intratracheal administration of <i>P</i>. <i>aeruginosa</i>. Since CLP mice died until 5 days, final survival data were given by subtracting lethality induced by CLP during the first assault. So we considered the remaining living mice as 100% for this second survival study. Either PBS (Sham n = 6, CLP n = 10) or <i>Pseudomonas aeruginosa</i> were instilled. For bacterial challenge, three doses were administered: 5.10⁶ CFU (Sham n = 6 and CLP n = 15), 2.10⁷ CFU (Sham n = 10 and CLP n = 17) and 10⁸ CFU (Sham n = 7 and CLP n = 20). Results are expressed as Kaplan Meier survival curves. p < 0.05 were considered statistically significant.