β₂-GPI and deglycosylated β₂-GPI inhibits the VEGF-induced tube formation in HAECs.

<p>(A) HAECs were seeded on the surface of a basement membrane extract and were treated with or without VEGF in combination with β₂-GPI at indicated concentrations. The results are representative of those observed in four separate experiments (×40). (B) The degree of tube formation in HAECs was estimated by the Metamorph tube formation module. Bar graphs represent the quantitative analysis of tube formation. (C) Images were taken in HAECs treated with or without VEGF in combination with β₂-GPI and deglycosylated β₂-GPI. The results are representative of those observed in four separate experiments. (D) The effect of β₂-GPI and deglycosylated β₂-GPI on tube formation in HAECs was estimated by the Metamorph tube formation module. Bar graphs represent the quantitative analysis of tube formation expressed as mean ± SEM and representative of more than three independent experiments. **<i>p</i> < 0.01 versus control; ^##<i>p</i> < 0.01 versus VEGF treatment alone.