Infectivity of C-Env virions, Env incorporation in virions produced by CD4+ T-cells and cell-to-cell transmission are lessened.

<p><b>(A and B) Infectivity of virions produced by HEK 293T cells (A) and CD4+ T-cells (B) in a single round TZM-bl assay.</b> TZM-bl cells (2x10⁴ cells/well) were infected with 2 ng/ml equivalent p24 of Env or EnvEC recombinant viruses produced from HEK 293T cells <b>(A)</b> or from CD4+ T-cells 5 days post infection <b>(B)</b>. Viral entry was monitored in cell lysates 48h post-infection. Infections were performed in triplicated wells. The means of three independent experiments with HEK293T cells and of 4 independent infections with CD4+ T cells from 4 different donors are shown. Error bars report standard deviation <b>C</b>. <b>Env incorporated in virions produced by CD4+ T-cells.</b> P24 and gp120 in day 5 supernatants of CD4+ T-cells were quantified by Western Blot. One representative experiment of two is shown. <b>D and E. Cell-to-cell transmission.</b> CD4+ T-cells were infected as above with 1 ng/ml equivalent p24. After 48 hours, cell were cocultured with TZM-bl cells in the presence of IDV to avoid any contribution of free virus. The next morning, MVC and AMD3100 were added to ensure single-round infection. Luciferase in TZM-bl cells was measured after 48 hours. <b>(D)</b> The mean and standard deviation of four independent experiments performed with CD4+ T-cells from four different donors are reported. <b>(E)</b> The same data is shown for each individual virus pair.