GSTA3 Attenuates Renal Interstitial Fibrosis by Inhibiting TGF-Beta-Induced Tubular Epithelial-Mesenchymal Transition and Fibronectin Expression - Fig 1

<p><b>A) Renals of UUO rats underwent display significant pathological changes and an increase in collagen I expression</b>. Sprague-Dawley rats were divided into 2 groups (5/group): Sham- operation (SHM) and UUO. All rats were sacrificed at day 14. renal cortex stained with HE, Masson’s trichrome or immunohistochemical for collagen I (×200). Semiquantitative analysis of tubulointerstitial damages as the degree of interstitial collagen deposition and collagen I expression in the renal cortex of rats from each group. Data expressed as means±S.E. *p<0.05 vs. SHM. <b>B) Expression of α-SMA protein was increased and that of E-cadherin protein was decreased in renal cortex of UUO rats.</b> The renal cortexes from different rats were lysed. The cell lysates were blotted with anti-α-SMA and anti-E-cadherin antibody. <b>C) Expression of α-SMA and Collagen I mRNA increased and that of E-cadherin mRNA decreased in renal cortex of UUO rats.</b> The mRNA from different rats was extracted, and the mRNA for α-SMA, E-cadherin, and Collagen I were detected by real-time PCR. Data expressed as means±S.E. *p<0.05 vs. SHM.