PpL induces the apoptosis of κ+ murine lymphoma B cells.

<p><b>A-B) PpL decreases the DNA content of κ+ murine lymphoma B cells</b>. LBK, A20 and LBO cells were incubated in the presence of PpL (100 μg/ml), OVA (100 μg/ml) or PBS. After 72 hs, cells were labeled with PI and the DNA content was measured by FACS. <b>A)</b> Representative overlaid histograms of PI staining. <b>B)</b> Mean ±SEM of three independent experiments <b>(</b>*** p<0.001 PpL vs PBS.) <b>C)</b> <i>In vivo</i> PpL increases the apoptosis of A20 cells. A20 cells stained with CFSE were inoculated in the footpad of BALB/c mice. Twelve hs later animals were inoculated with PpL, OVA or PBS. After 12 hs popliteal lymph nodes were excised and cells were stained with Annexin V. The percentage of Annexin V+ within CFSE+ cells is shown. Data are presented as the mean ± SEM of three independent experiments, (** p<0.01 PpL vs PBS).